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trypsin


Term: trypsin
Literally meaning: “protein of rubbing
Origin: Greek
τρίβω/trivo (=rub, powder, grind) > (simple past) ἔτριψα /etripsα
Coined/History
The enzyme named in 1876 by Kuhne who first described an proteolytic activity of pancreatic extract by comparing trypsin with pepsin. In 1931 J.H Northrop purified and crystallized trypsin shortly after first purifying pepsin (1930) and in addition to chemotrypsin (M Kunitz and JH Northrop).
 
Definition
Trypsin is a proteolytic enzyme that cleaves the peptide bonds at carboxy site of residues of lysine and arginine, except when either is followed by proline. Trypsin is active in the duodenum and it is produced by the pancreas after the proteolysis of proenzyme trypsinogen. Today, trypsin is used in routine protocols for cell and tissue cultures by the process of  “trypsinization”.  

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